1. HUMAN REPRODUCTION PHYSIOLOGY.
   1. Pelvic anatomy
   2. Steroid hormones
   3. Effects of ageing
   4. Neuroendocrinology of the reproductive axis
   5. Folliculogenesis and ovulation
   6. Hormones of the reproductive system
   7. Endocrinology of the testicle
   8. Obesity and endocrine axes.
   9. Menstrual vs. Estrous cycle.

1. OOGENESIS, SPERMATOGENESIS AND THEIR DEFICIENCIES
   1. Biological relevance of the meiosis
   2. Ovogenesis
   3. Folliculogenesis
   4. Ovulation induction
   5. Spermatogenesis and sperm production
   6. Markers of sperm quality
   7. Factors affecting sperm quality
   8. Markers of oocyte quality
   9. Oocyte maturation
   10. in vitro gamete derivation from human embryonic stem cells.

1. FERTILIZATION, EMBRYO DEVELOPMENT AND TECHNIQUES TO OBTAIN IN VIVO PRODUCED EMBRYOS
   1. Fertiization and embryo development
   2. Gamete maturation
   3. Fertilization, fertilization anomalies and uniparental inheritances
   4. Embryo development
   5. Main landmarks in embryo development.

1. STERILITY.
   1. Polycystic ovary syndrome
   2. Sterility
   3. Cervical and immune sterility
   4. Uterine and tubal sterility
   5. Endometriosis
   6. Artificial insemination and in vitro fertilization
   7. Repeated miscarriage
   8. Egg donation
   9. Ovulation induction
   10.Special cases in IVF
   11. Ovarian and endometrial manipulation

1. BASIC RESEARCH IN ASSISTED REPRODUCTION
   1. Basic research in assisted reproduction
   2. Nucleic acids
   3. Separation techniques
   4. Microscopy
   5. vitro studies
   6. Microarrays
   7. Bioinformatics
   8. ERA
   9. Massive sequencing
   10.Culture of human granulosa cells
   11. Animal models for the study of the ovarian
   12. hyperstimulation syndrome and endometriosis
   13. Lipidomics

1. BASIC TECHNIQUES IN ASSISTED REPRODUCTION
   1. Structure of an AR unit
   2. History of the ART
   3. Diagnostic procedures in the clinical analysis laboratory
   4. Low complexity techniques
   5. High complexity procedures
   6. Donation of gametes
   7. Applied molecularbiology techniques
   8. Research in AR

7. ANDROLOGY LABORATORY

1. The testicle and the spermatogenesis
2. Semen analysis
3. Sperm preparation techniques for conjugal artificial insemination
4. Application of image analysis in sperm
5. Sperm freezing techniques
6. Selection of donors
7. Management and control of the sperm bank
8. Reproductive treatment in HIV-seropositive males
9. Flow cytometry in sperm
10. Sperm DNA fragmentation
11. Methods for an objective selection
12. Microarrays
13. Other molecular markers of sperm quality.

8. IN VITRO FERTILIZATION LABORATORY

1. Embryology Laboratory
2. Oocyte retrieval and insemination
3. Micromanipulation techniques
4. Evaluation of fertilization
5. Oocyte dysmorphisms
6. Standard embryo culture
7. Cryopreservation of oocytes and embryos
8. Quality management in the embryology laboratory

9. SEX DETERMINATION TECHNIQUES

1. Animal sexing
2. Analysis of the sperm DNA content
3. Legislation according to sex determination
4. Molecular bases of sex determination
5. Sexual differentiation in humans
6. Advantages of sexual reproduction
7. Relevance of preconception and preimplantation 8. sex selection in humans
9. Alterations in the sex ratio

10. IN VITRO PRODUCTION OF EMBRYOS. EMBRYO CULTURE

1. Cell culture
2. Culture media
3. Embryo development
4. Types of prolonged cultures
5. The blastocyst
6. Application of embryo co-culture in research

11. CRYOBIOLOGY. PRESERVATION OF MATERNAL AND PATERNAL INHERITANCE. EMBRYO PRESERVATION.

1. Principles of physics
2. Water dynamics
3. Dynamics of water with solutes
4. Dynamics of solution with cells
5. Cryoprotectants
6. Cryopreservation: balance
7. Cryopreservation: non-balance
8. Cryopreservation of maternal and paternal inheritance
9. Cryopreservation of ovarian tissue

12. EMBRYO QUALITY IMPROVEMENT

Morphological parameters
2. Non-morphological parameters
3. Improvement of embryo quality
4. Intrinsecal factors affecting embryo quality: effect of the spermatozoon
5. Intrinsecal factors affecting embryo quality: effect of the oocyte
6. Extrinsecal factors affecting embryo quality
7. Ex vivo culture conditions
8. Nuclear and cytoplasmic transfer
9. Assisted hatching and aspiration of fragments

13. MULTIPLICATION OF EMBRYOS AND GAMETES

1. Embryo cloning without reprogramming
2. Embryo cloning by means of nuclear transfer
3. Methodology
4. Reversibility of the differentiated status
5. Applications
6. Multiplication of gametes

14. PREIMPLANTATION GENETIC SCREENING LABORATORY

1. Origin of aneuploidies
2. Chromosomal alterations in the infertile couple and genetic counseling
3. Genetic studies in the infertile couple
4. FISH
5. Embryo biopsy
6. PGD for the screening of aneuploidies
7. PGD for structural chromosomal anomalies
8. Relation between embryo morphology and chromosomal anomalies
9. Correction of aneuploidies
10. PGD for the screening of monogenic diseases
11. PGD for HLA matching
12. PGD by means of massive sequencing

15. STEM CELLS

1. Stem cells
2. Pluripotent cell. Human Embryonic Stem Cells:
Derivation, culture and characterization
3. Reprogramming: iPSC
4. Cancer stem cells
5. Adult stem cells
6. Umbilical cord blood bank
7. Adult stem cells in human testicles
8. Adult stem cells in human ovaries
9. Somatic stem cells in the endometrium
10. Somatic stem cells in the myometrium
11. Differentiation of gametes
12. Bioethics and legislation

16. LEGAL ETHICAL CONSIDERATIONS OF ASSISTED HUMAN REPRODUCTION.

Legal ethical considerations of assisted human reproduction.

1. Initial conceps

2. Ethical aspects of the application of the TRA: bioethics

3. Law of autonomy of the patient

4. Research in assisted reproduction.

17. FINAL MASTER WORK